Anti-DDB1 with anti-GAPDH internal loading control antibody (ab75393)
Key features and details
- Rabbit polyclonal to DDB1 with anti-GAPDH internal loading control
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DDB1 with anti-GAPDH internal loading control antibody -
Description
Rabbit polyclonal to DDB1 with anti-GAPDH internal loading control -
Host species
Rabbit -
Specificity
ab75393 contains 2 antibodies, generated by raising against an DDB1 immunogen and spiking with anti-GAPDH loading control. -
Tested applications
Suitable for: WBmore details
Unsuitable for: ChIP,Flow Cyt,ICC or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Elephant
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Immunogen
Synthetic peptide corresponding to Human DDB1 with anti-GAPDH internal loading control aa 1050 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab138814) -
Positive control
- This cocktail gave a positive signal in the following whole cell lysates: HeLa; HeLa Irradiated; MCF7; T47D; HepG2; Jurkat; Caco2.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab75393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 37, 127 kDa (predicted molecular weight: 37, 127 kDa). ab75393 contains 2 antibodies, generated by raising against an DDB1 immunogen and spiking with anti-GAPDH loading control. Application notesIs unsuitable for ChIP,Flow Cyt,ICC or IHC-P.Target
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Function
Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the DDB1 family. -
Post-translational
modificationsPhosphorylated by ABL1.
Ubiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis. -
Cellular localization
Cytoplasm. Nucleus. Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage. - Information by UniProt
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Database links
- Entrez Gene: 1642 Human
- SwissProt: Q16531 Human
- Unigene: 290758 Human
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Alternative names
- Damage-specific DNA-binding protein 1 antibody
- DDB p127 subunit antibody
- Ddb1 antibody
see all
Images
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Western blot - Anti-DDB1 with anti-GAPDH internal loading control antibody (ab75393)All lanes : Anti-DDB1 with anti-GAPDH internal loading control antibody (ab75393) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Hela Whole Cell Lysate - UV Irradiated
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 4 :T47D whole cell lysate (ab14899)
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 7 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37, 127 kDa
Observed band size: 127,37 kDa why is the actual band size different from the predicted?
Additional bands at: 32 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
The 127 and 37 kDa bands correlate to DDB1 and GAPDH, respectively.
Datasheets and documents
References (0)
ab75393 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-DDB1 with anti-GAPDH internal loading control antibody (ab75393)All lanes : Anti-DDB1 with anti-GAPDH internal loading control antibody (ab75393) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Hela Whole Cell Lysate - UV Irradiated
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 4 :T47D whole cell lysate (ab14899)
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 7 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37, 127 kDa
Observed band size: 127,37 kDa why is the actual band size different from the predicted?
Additional bands at: 32 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
The 127 and 37 kDa bands correlate to DDB1 and GAPDH, respectively.