Recombinant human PDGF B protein (His tag) (ab221402)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus, Avi tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: 5% Trehalose
Acetonitrile and TFA.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with 100mM acetic acid to a concentration of 100 µg/ml.
Images
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Functional Studies - Recombinant human PDGF B protein (His tag) (ab221402)
Immobilized Human PDGF-BB, His Tag at 5 µg/mL (100 µl/well) can bind ab221402 with a linear range of 0.02-1.2 µg/mL.
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SDS-PAGE - Recombinant human PDGF B protein (His tag) (ab221402)SDS-PAGE using ab221402. The protein migrates as 17 kDa under reducing (Lane 1) conditions and 35 kDa under non-reducing (Lane 2) conditions.
