Recombinant human PD-L2 protein (ab191645)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 0.61% Tris, 0.75% Glycine, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
Images
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Biological activity - Recombinant human PD-L2 protein (ab191645)
Immobilized cell surface PD-1 (5x104 of cells per well) can bind Human PD-L2, Fc Tag ab191645 with an EC50 of 0.018 μg/mL.
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Flow Cytometry - Recombinant human PD-L2 protein (ab191645)Flow Cytometry assay shows that recombinant Human PD-L2, Fc Tag ab191645 can bind to 293 cell overexpressing human PD-1. The concentration of PD-L2 used is 1 μg/mL
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Biological activity - Recombinant human PD-L2 protein (ab191645)Loaded Human PD-1, His Tag, low endotoxin on HIS1K Biosensor, can bind Human PD-L2, Fc Tag ab191645 with an affinity constant of 15.7 nM as determined in BLI assay
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Biological activity - Recombinant human PD-L2 protein (ab191645)Loaded Human PD-1, His Tag (ab174035), can bind Human PD-L2, Fc Tag ab191645 with an affinity constant of 16.3 nM as determined in BLI assay
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HPLC - Recombinant human PD-L2 protein (ab191645)The purity of ab191645 was greater than 90% as determined by SEC-HPLC.
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SDS-PAGE - Recombinant human PD-L2 protein (ab191645)DTT-reduced (+) SDS-PAGE staining ab191645 overnight with Coomassie Blue.
