Recombinant human PD-L2 protein (Fc Chimera Active) (ab221305)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 5% Trehalose, 0.75% Glycine, 0.61% Tris buffer, Sodium chloride, L-Arginine
Lyophilized from 0.22 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
Images
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Functional Studies - Recombinant human PD-L2 protein (Fc Chimera Active) (ab221305)FACS analysis shows that the binding of Human PD-L2, mouse IgG1 Fc Tag to 293 overexpressing PD-1 was inhibited by increasing concentration of neutralizing anti-PD-1 antibody. The concentration of PD-L2 used is 0.1 µg/ml. The IC50 is 1.3 µg/mL.
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Functional Studies - Recombinant human PD-L2 protein (Fc Chimera Active) (ab221305)Flow Cytometry assay shows that recombinant Human PD-L2, mouse IgG1 Fc Tag can bind to 293 cell overexpressing human PD-1. The concentration of PD-L2 used is 0.1 µg/ml. -
Functional Studies - Recombinant human PD-L2 protein (Fc Chimera Active) (ab221305)Immobilized Human PD-1, His Tag at 2 µg/mL (100 µL/well) can bind Human PD-L2, Mouse IgG1 Fc Tag with a linear range of 10-156 ng/mL. -
SDS-PAGE - Recombinant human PD-L2 protein (Fc Chimera Active) (ab221305)SDS-PAGE - Human PD-L2 protein (Fc Chimera Active) (ab221305).
ab221305 on SDS-PAGE under reducing (R) conditions.
The gel was stained overnight with Coomassie Blue.
