Recombinant human PCK1/PEPC protein (Active) (ab229508)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human PCK1/PEPC protein (Active)
See all PCK1/PEPC proteins and peptides -
Biological activity
Specific activity >100 mU/mg.
One unit is the amount of enzyme that transfers a phosphate group from PEP to GDP and generates 1.0 µmol of pyruvate per minute at pH 7.5 at 37oC
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Purity
> 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
PPQLQNGLNLSAKVVQGSLDSLPQAVREFLENNAELCQPDHIHICDGSEE ENGRLLGQMEEEGILRRLKKYDNCWLALTDPRDVARIESKTVIVTQEQRD TVPIPKTGLSQLGRWMSEEDFEKAFNARFPGCMKGRTMYVIPFSMGPLGS PLSKIGIELTDSPYVVASMRIMTRMGTPVLEAVGDGEFVKCLHSVGCPLP LQKPLVNNWPCNPELTLIAHLPDRREIISFGSGYGGNSLLGKKCFALRMA SRLAKEEGWLAEHMLILGITNPEGEKKYLAAAFPSACGKTNLAMMNPSLP GWKVECVGDDIAWMKFDAQGHLRAINPENGFFGVAPGTSVKTNPNAIKTI QKNTIFTNVAETSDGGVYWEGIDEPLASGVTITSWKNKEWSSEDGEPCAH PNSRFCTPASQCPIIDAAWESPEGVPIEGIIFGGRRPAGVPLVYEALSWQ HGVFVGAAMRSEATAAAEHKGKIIMHDPFAMRPFFGYNFGKYLAHWLSMA QHPAAKLPKIFHVNWFRKDKEGKFLWPGFGENSRVLEWMFNRIDGKASTK LTPIGYIPKEDALNLKGLGHINMMELFSISKEFWEKEVEDIEKYLEDQVN ADLPCEIEREILALKQRISQM -
Predicted molecular weight
71 kDa including tags -
Amino acids
2 to 622 -
Tags
His tag N-Terminus
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Proprietary buffer.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute to 2 mg/ml in 30% glycerol.
Images
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SDS-PAGE - Recombinant human PCK1/PEPC protein (Active) (ab229508)
4-20% SDS-PAGE analysis of ab229508 under reducing conditions and stained with Coomassie Blue.
Lane 1: 0.5 µg; Lane 2: 1 µg; Lane 3: 1.5 µg.
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Functional Studies - Recombinant human PCK1/PEPC protein (Active) (ab229508)Activity of ab229508 was measured by a multi-steps assay. In this assay, PCK1/PEPC converts phosphoenolpyruvate into oxalacetate, then coupling with series of enzymatic reactions to generate pyruvate, which in turn reacts with a probe and developer to generate color (OD570 nm). Legend on the right indicated the amount of ab229508.
