Recombinant human PAK2 protein (ab95310)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies
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Product name
Recombinant human PAK2 protein
See all PAK2 proteins and peptides -
Biological activity
86 nmoles/min/µg Analysis of enzymatic activity: 1. Different concentrations of PAK2 were incubated in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 20) at room temperature for 1 hour. 2. Developer solution was added to reaction and reaction was stopped after 1h of incubation at RT. 3. Fluorescence was detected using ?exc=460±40 nm and ?em=528±20 nm filters. -
Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
33 kDa -
Amino acids
227 to 524
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human PAK2 protein (ab95310)
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SDS-PAGE - Recombinant human PAK2 protein (ab95310)SDS-PAGE analysis of PAK2 (ab95310) and BSA
1, 2, 3 : ab95310 at 6, 60 and 120ug/ml
4, 5, 6 : BSA at 5, 50, 100ug/ml
