Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RAET1E antibody [EPR17443] - BSA and Azide free (ab251318)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17443] to RAET1E - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RAET1E antibody [EPR17443] - BSA and Azide free
    See all RAET1E primary antibodies

  • Description

    Rabbit monoclonal [EPR17443] to RAET1E - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251318 is the carrier-free version of ab200662 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251318 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as ULBP4

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Clonality

    Monoclonal

  • Clone number

    EPR17443

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-RAET1E antibody [EPR17443] - BSA and Azide free (ab251318)
    Western blot - Anti-RAET1E antibody [EPR17443] - BSA and Azide free (ab251318)
    All lanes : Anti-RAET1E antibody [EPR17443] (ab200662) at 1/5000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma) cell lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
    Lane 3 : MCF-7 (Human breast adenocarcinoma cell line) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa
    Observed band size: 30 kDa


    Exposure time: 30 seconds


    This data was developed using ab200662, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-RAET1E antibody [EPR17443] - BSA and Azide free (ab251318)
    Western blot - Anti-RAET1E antibody [EPR17443] - BSA and Azide free (ab251318)
    Anti-RAET1E antibody [EPR17443] (ab200662) at 1/1000 dilution + Human fetal brain tissue lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 30 kDa
    Observed band size: 30 kDa


    Exposure time: 1 minute


    This data was developed using ab200662, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-RAET1E antibody [EPR17443] - BSA and Azide free (ab251318)
    Western blot - Anti-RAET1E antibody [EPR17443] - BSA and Azide free (ab251318)
    All lanes : Anti-RAET1E antibody [EPR17443] (ab200662) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cells) cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) cell lysate
    Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa
    Observed band size: 30 kDa


    Exposure time: 10 seconds


    This data was developed using ab200662, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-RAET1E antibody [EPR17443] - BSA and Azide free (ab251318)
    Immunoprecipitation - Anti-RAET1E antibody [EPR17443] - BSA and Azide free (ab251318)
    This data was developed using ab200662, the same antibody clone in a different buffer formulation.RAET1E was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab200662 at 1/100 dilution. Lane 1: HeLa whole cell lysate 10 µg (Input). Lane 2: ab200662 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200662 in HeLa whole cell lysate. Western blot was performed using ab200662 at 1/2000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  • Anti-RAET1E antibody [EPR17443] - BSA and Azide free (ab251318)
    Anti-RAET1E antibody [EPR17443] - BSA and Azide free (ab251318)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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