Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Glucosidase 2 subunit beta antibody [EPR8047] - BSA and Azide free (ab248297)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8047] to Glucosidase 2 subunit beta - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Glucosidase 2 subunit beta antibody [EPR8047] - BSA and Azide free
    See all Glucosidase 2 subunit beta primary antibodies

  • Description

    Rabbit monoclonal [EPR8047] to Glucosidase 2 subunit beta - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HEK293T, Daudi, HeLa, Jurkat, A431, and K562 cell lysates; Human brain tissue; HeLa cells.

  • General notes

    ab248297 is the carrier-free version of ab129098 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248297 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Glucosidase 2 subunit beta antibody [EPR8047] - BSA and Azide free (ab248297)
    Western blot - Anti-Glucosidase 2 subunit beta antibody [EPR8047] - BSA and Azide free (ab248297)
    All lanes : Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098) at 1/10000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : PRKCSH knockout HEK-293T cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 59 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab129098).

    Lanes 1- 4: Merged signal (red and green). Green - ab129098 observed at 80 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab129098 was shown to react with PRKCSH in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266770 (knockout cell lysate ab257608) was used. Wild-type HEK-293T and PRKCSH knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab129098 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucosidase 2 subunit beta antibody [EPR8047] - BSA and Azide free (ab248297)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucosidase 2 subunit beta antibody [EPR8047] - BSA and Azide free (ab248297)
    This data was developed using ab129098, the same antibody clone in a different buffer formulation.ab129098, at a 1/250 dilution, staining Glucosidase 2 subunit beta in paraffin embedded Human brain tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-Glucosidase 2 subunit beta antibody [EPR8047] - BSA and Azide free (ab248297)
    Western blot - Anti-Glucosidase 2 subunit beta antibody [EPR8047] - BSA and Azide free (ab248297)
    All lanes : Anti-Glucosidase 2 subunit beta antibody [EPR8047] (ab129098) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 59 kDa



    This data was developed using ab129098, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-Glucosidase 2 subunit beta antibody [EPR8047] - BSA and Azide free (ab248297)
    Immunocytochemistry - Anti-Glucosidase 2 subunit beta antibody [EPR8047] - BSA and Azide free (ab248297)
    This data was developed using ab129098, the same antibody clone in a different buffer formulation.ab129098, at a 1/250 dilution, staining Glucosidase 2 subunit beta in HeLa cells by Immunofluorescence.
  • OI-RD Scanning - Anti-Glucosidase 2 subunit beta antibody [EPR8047] - BSA and Azide free (ab248297)
    OI-RD Scanning - Anti-Glucosidase 2 subunit beta antibody [EPR8047] - BSA and Azide free (ab248297)
    This data was developed using ab129098, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Glucosidase 2 subunit beta antibody [EPR8047] - BSA and Azide free (ab248297)
    Anti-Glucosidase 2 subunit beta antibody [EPR8047] - BSA and Azide free (ab248297)

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