Mouse MER ELISA Kit (ab210572)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 27.8 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse MER ELISA Kit
See all MERTK kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.6% Inter-assay Sample n Mean SD CV% Serum 3 8.9% -
Sample type
Cell culture supernatant, Serum, Plasma, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
27.8 pg/ml -
Range
156 pg/ml - 10000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 103 99% - 105% Serum 108 102% - 117% Tissue Extracts 103 95% - 109% Hep Plasma 105 100% - 115% EDTA Plasma 110 103% - 115% Cit plasma 100 98% - 102% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse MER ELISA Kit (ab210572) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MER protein in plasma, serum, tissue extracts, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MER with 27.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
MER is a receptor tyrosine kinase that regulates a variety of cellular processes, including cell survival, migration, differentiation and efferocytosis along with the immune response.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse MER Capture Antibody 1 x 600µl 10X Mouse MER Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Mouse MER Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
In case of filovirus infection, seems to function as a cell entry factor. -
Tissue specificity
Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. -
Involvement in disease
Defects in MERTK are the cause of retinitis pigmentosa type 38 (RP38) [MIM:613862]. RP38 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- c MER
- c mer proto oncogene tyrosine kinase
- c-mer
see all -
Database links
- Entrez Gene: 17289 Mouse
- SwissProt: Q60805 Mouse
- Unigene: 239655 Mouse
Images
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Other - Mouse MER ELISA Kit (ab210572)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse MER standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse MER standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native MER in mouse serum and plasma samples.The concentrations of MER were measured in duplicates, interpolated from the MER standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 20%, plasma (citrate) 20%, plasma (heparin) 20% and plasma (EDTA) 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MER concentration was determined to be 27.7 ng/mL in serum, 28.6 ng/mL in plasma (citrate), 37.1 ng/mL in plasma (heparin) and 37.8 ng/mL in plasma (EDTA).
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Interpolated concentrations of native MER in mouse pancreas extract based on a 1,000 µg/mL extract load.The concentrations of MER were measured in duplicate and interpolated from the MER standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MER concentration was determined to be 3.64 ng/mL in pancreas extract.
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Sandwich ELISA - Mouse MER ELISA Kit (ab210572)To learn more about the advantages of recombinant antibodies see here.
