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Human MAP3K14 (NFkB Inducing Kinase NIK) knockout A549 cell pellet (ab279186)

Overview

  • Product name

    Human MAP3K14 (NFkB Inducing Kinase NIK) knockout A549 cell pellet

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    A549

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 34 bp deletion in exon2 and 7 bp deletion in exon2.

  • Passage number

  • Knockout validation

    Sanger Sequencing

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Properties

Target

  • Function

    Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner.

  • Tissue specificity

    Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon.

  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Autophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization.
    Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Alternative names

    • MAP3K14
    • FTDCR1B
    • HS
    • HsNIK
    • M3K14_HUMAN
    • Map3k14
    • Mitogen activated protein kinase kinase kinase 14
    • Mitogen-activated protein kinase kinase kinase 14
    • NF kappa beta inducing kinase
    • NF-kappa-beta-inducing kinase
    • NIK
    • Serine/threonine-protein kinase NIK
    see all

Properties

Images

  • Sanger Sequencing - Human MAP3K14 (NFkB Inducing Kinase NIK) knockout A549 cell pellet (ab279186)
    Sanger Sequencing - Human MAP3K14 (NFkB Inducing Kinase NIK) knockout A549 cell pellet (ab279186)

    Allele-1: 34 bp deletion in exon2

     

  • Sanger Sequencing - Human MAP3K14 (NFkB Inducing Kinase NIK) knockout A549 cell pellet (ab279186)
    Sanger Sequencing - Human MAP3K14 (NFkB Inducing Kinase NIK) knockout A549 cell pellet (ab279186)

    Allele-2: 7 bp deletion in exon2

     

  • Sanger Sequencing - Human MAP3K14 (NFkB Inducing Kinase NIK) knockout A549 cell pellet (ab279186)
    Sanger Sequencing - Human MAP3K14 (NFkB Inducing Kinase NIK) knockout A549 cell pellet (ab279186)

    Allele-3: 1 bp insertion in exon2

     

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