Recombinant Human Leptin protein (ab51273)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: WB, SDS-PAGE
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Product name
Recombinant Human Leptin protein
See all Leptin proteins and peptides -
Purity
> 95 % SDS-PAGE.
Recombinant human Leptin was overexpressed as insoluble protein aggregate in E.coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL QGSLQDMLWQ LDLSPGC
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.40
Constituent: PBS
Images
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Sandwich ELISA - Recombinant Human Leptin protein (ab51273)Standard curve for Leptin (Analyte: ab51273); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [BDI170] to Leptin (ab20101) at 5µg/ml and Detector Antibody Rabbit polyclonal to Leptin (ab7208) at 0.5µg/ml. Concentration of Mouse monoclonal [BDI170] to Leptin (ab20101) may vary from lot to lot; please use this curve as guideline.
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SDS-PAGE - Recombinant Human Leptin protein (ab51273)ab51273 on 14% SDS-PAGE -
Western blot - Recombinant Human Leptin protein (ab51273)
