Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant human Parathyroid Hormone protein (ab201365)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 97% SDS-PAGE
  • Active: Yes
  • Suitable for: Functional Studies, HPLC, SDS-PAGE

Description

  • Product name

    Recombinant human Parathyroid Hormone protein
    See all Parathyroid Hormone proteins and peptides

  • Biological activity

    Fully biologically active when compared to standard.

    The ED50 as determined by its ability to induce cAMP accumulation in murine MC3T3E1 cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 104 IU/mg.

  • Purity

    > 97 % SDS-PAGE.
    > 97 % by HPLC analysis.

  • Expression system

    Escherichia coli

  • Accession

    P01270

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      SVSEIQLMHNLGKHLNSMERVEWLRKKLQDVHNFVALGAPLAPRDAGSQR PRKKEDNVLVESHEKSLGEADKADVNVLTKAKSQ

    • Predicted molecular weight

      9 kDa

    • Amino acids

      32 to 115

    • Additional sequence information

      Single non-glycosylated polypeptide chain containing 84 amino acids. 15N Stable Isotope labeled. This product is for the mature full length protein. The signal peptide and propeptide are not included.

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C long term. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituent: 100% PBS

    Lyophilized from a 0.2µm filtered solution.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Briefly centrifuge the vial prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at

    General Info

    • Alternative names

      • hPTH
      • Parathormone
      • Parathyrin
      • Parathyroid hormone
      • Parathyroid hormone 1
      • Prepro PTH
      • Preproparathyroid hormone
      • PTH
      • PTH1
      • PTH1 receptor
      • PTH1R
      • PTHR
      • PTHR1
      • PTHY_HUMAN
      see all

    • Function

      PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.

    • Involvement in disease

      Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.

    • Sequence similarities

      Belongs to the parathyroid hormone family.

    • Cellular localization

      Secreted.
    • Information by UniProt

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

References (0)

Publishing research using ab201365? Please let us know so that we can cite the reference in this datasheet.

ab201365 has not yet been referenced specifically in any publications.

Images

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Recombinant human Parathyroid Hormone protein (ab201365)

Get resources and offers direct to your inbox Sign up