Recombinant human KDM4E protein (ab196415)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 37% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
-
Product name
Recombinant human KDM4E protein -
Biological activity
Specific activity: 0.14 pmole/min/μg.
10µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl, 500 µM α-ketoglutarate, 25 µM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and ab196415 (100 – 400 ng) added to the wells. Add antibody against demethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by detection.
-
Purity
>= 37 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
KSVHSSPQNTSHTIMTFYPTMEEFADFNTYVAYMESQGAHQAGLAKVIPP KEWKARQMYDDIEDILIATPLQQVTSGQGGVFTQYHKKKKAMRVGQYRRL ANSKKYQTPPHQNFADLEQRYWKSHPGNPPIYGADISGSLFEESTKQWNL GHLGTILDLLEQECGVVIEGVNTPYLYFGMWKTTFAWHTEDMDLYSINYL HFGEPKTWYVVPPEHGQHLERLARELFPDISRGCEAFLRHKVALISPTVL KENGIPFNCMTQEAGEFMVTFPYGYHAGFNHGFNCAEAINFATPRWIDYG KMASQCSCGESTVTFSMDPFVRIVQPESYELWKHRQ -
Predicted molecular weight
65 kDa including tags -
Amino acids
2 to 337 -
Tags
GST tag N-Terminus -
Additional sequence information
NM_001161630.
-
Preparation and Storage
- JMJD2E
- KDM4D-like protein
- KDM4DL
Images
-
SDS-PAGE - Recombinant human KDM4E protein (ab196415)
10% SDS-PAGE analysis of ab196415 with Coomassie staining.
-
Functional Studies - Recombinant human KDM4E protein (ab196415)Activity assay of ab196415.
