Anti-PDCD4 antibody (ab51495)
Key features and details
- Rabbit polyclonal to PDCD4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-PDCD4 antibody
See all PDCD4 primary antibodies -
Description
Rabbit polyclonal to PDCD4 -
Host species
Rabbit -
Specificity
ab51495 reacts with PDCD4 protein that is either phosphorylated or non phosphorylated at Ser 457. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human, Recombinant fragment
Predicted to work with: Rat, Xenopus laevis
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Immunogen
A synthetic peptide corresponding to amino acids near the carboxyl terminus of human PDCD4 protein
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Positive control
- WB: 293 HEK cells, Mouse N2A cell Lysate. IHC-P: Mouse colon tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab51495 was affinity purified from monospecific antiserum by immunoaffinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PDCD4 antibody (ab51495)All lanes : Anti-PDCD4 antibody (ab51495) at 1/5000 dilution
Lane 1 : recombinant PDCD4
Lane 2 : 293 HEK cells treated with TPA and MG132.
Predicted band size: 52 kDa
Observed band size: 52 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 antibody (ab51495)ab51495, at 1/100 dilution, staining human PDCD4 in paraformaldehyde fixed (4%), paraffin embedded mouse colon tissue by Immunohistochemistry. -
Western blot - Anti-PDCD4 antibody (ab51495) This image is courtesy of an anonymous AbreviewAll lanes : Anti-PDCD4 antibody (ab51495) at 1/1000 dilution
Lane 1 : Human HEK cell Lysate at 30 ug/ml
Lane 2 : Mouse N2A cell Lysate at 30 ug/ml
Secondary
All lanes : Li-Cor IRDye®800CW-conjugated Goat anti rabbit
Predicted band size: 52 kDa
13% SDS-Page gel used, Blocking done for 1 hour at RT, Primary Incubation overnight at 4°C.
