Recombinant human IL-15 protein (Active) (ab259403)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: HPLC, MS, SDS-PAGE, Cell Culture, FRET
Preparation and Storage
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Alternative names
- IL 15
- IL-15
- IL15
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Function
Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha. -
Tissue specificity
Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15-S21AA is preferentially expressed in tissues such as testis and thymus. -
Sequence similarities
Belongs to the IL-15/IL-21 family. -
Cellular localization
Secreted and Cytoplasm. Nucleus. IL15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components. - Information by UniProt
Images
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Functional Studies - Recombinant human IL-15 protein (Active) (ab259403)
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant proliferation of CTLL-2 cells is 0.97 ng/mL corresponding to a Specific Activity of 1.03 x 106 IU/mg.
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SDS-PAGE - Recombinant human IL-15 protein (Active) (ab259403)SDS-PAGE analysis of ab259403.
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HPLC - Recombinant Human IL-15 protein (Active) (ab259403)Purity: ≥95%
The spectrum was recorded using a 1260 Infinity II HPLC system with DAD and a MabPac RP column (3.0x100 mm, 4 µm). 5 µL of purified protein was injected and the gradient run from 80 % water:TFA (99.9:0.1 v/v) and 20 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) to 20 % water:TFA (99.9:0.1 v/v) and 80 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 3 min. Flow rate was 0.5 mL/min and the column compartment temperature was 50 °C.
Purified IL-15 is known to accumulate heterogeneities due to deamidation, while retaining its activity. (Nellis, David F., et al. "Characterization of recombinant human IL-15 deamidation and its practical elimination through substitution of asparagine 77." Pharmaceutical research 29.3 (2012): 722-738.)
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Mass Spectrometry - Recombinant Human IL-15 protein (Active) (ab259403)M +1.4 Da (calc. mass 12830.6 Da)
The spectrum was recorded with a 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) and a MabPac RP column (42.1x50 mm, 4 µm, Thermo Scientific). 5 µL of purified protein was injected and the gradient run from 85 % water:FA (99.9:0.1 v/v) and 15 % acetonitrile:FA (90:9.9:0.1 v/v/v) to 55 % water:FA (99.9:0.1 v/v) and 45 % acetonitrile:FA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 2.5 min. Flow rate was 0.4 mL/min and the column compartment temperature was 60 °C. Data was analysed and deconvoluted using the Bioconfirm software (Agilent Technologies).
