Recombinant human IL-2 Receptor alpha protein (Active) (ab221397)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Preparation and Storage
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Images
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SDS-PAGE - Recombinant human IL-2 Receptor alpha protein (Active) (ab221397)
SDS-PAGE of reduced ab221397 stained overnight with Coomassie Blue. The protein migrates as 34-46 kDa under reducing conditions due to glycosylation.
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Functional Studies - Recombinant human IL-2 Receptor alpha protein (Active) (ab221397)Immobilized Biotinylated Human IL-2, Fc,Avitag at 1 µg/mL (100 µL/well) on streptavidin precoated (0.2 µg/well) plate, can bind ab221397 with a linear range of 1-39 ng/mL.
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Functional Studies - Recombinant human IL-2 Receptor alpha protein (Active) (ab221397)Ab221397 captured on CM5 chip via anti-His antibody, can bind Human IL-2, Tag Free with an affinity constant of 29.9 nM as determined in a SPR assay (Biacore T200).
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Functional Studies - Recombinant human IL-2 Receptor alpha protein (Active) (ab221397)Loaded ab221397 on HIS1K Biosensor, can bind Human IL-2, Tag Free with an affinity constant of 18 nM as determined in BLI assay (ForteBio Octet Red96e).
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Functional Studies - Recombinant human IL-2 Receptor alpha protein (Active) (ab221397)Ab221397 inhibits the IL-2 dependent proliferatino of Mo7e cells. The EC50 for this effect is 0.35-0.77 µg/mL.
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