Recombinant human HDAC6 (mutated H611A) protein (Active) (ab198058)
Key features and details
- Expression system: Baculovirus
- Purity: >= 79% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
-
Product name
Recombinant human HDAC6 (mutated H611A) protein (Active)
See all HDAC6 proteins and peptides -
Biological activity
Specific Activity: 7.5 pmole/min
Assay conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC substrate 3, and HDAC6 H611A (15.6 - 1000ng). Incubation condition: 30 min at 37°C followed by HDAC developer for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.
-
Purity
>= 79 % SDS-PAGE.
Protein was purified by affinity chromatography and gel filtration. -
Expression system
Baculovirus -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDPMGHHHHHHGRRRASVAAGILVPRGSPGLD GIYARGIQMTSTGQDSTTTRQRRSRQNPQSPPQDSSVTSKRNIKKGAVPR SIPNLAEVKKKGKMKKLGQAMEEDLIVGLQGMDLNLEAEALAGTGLVLDE QLNEFHCLWDDSFPEGPERLHAIKEQLIQEGLLDRCVSFQARFAEKEELM LVHSLEYIDLMETTQYMNEGELRVLADTYDSVYLHPNSYSCACLASGSVL RLVDAVLGAEIRNGMAIIRPPGHHAQHSLMDGYCMFNHVAVAARYAQQKH RIRRVLIVDWDVHHGQGTQFTFDQDPSVLYFSIHRYEQGRFWPHLKASNW STTGFGQGQGYTINVPWNQVGMRDADYIAAFLHVLLPVALEFQPQLVLVA AGFDALQGDPKGEMAATPAGFAQLTHLLMGLAGGKLILSLEGGYNLRALA EGVSASLHTLLGDPCPMLESPGAPCRSAQASVSCALEALEPFWEVLVRST ETVERDNMEEDNVEESEEEGPWEPPVLPILTWPVLQSRTGLVYDQNMMNH CNLWDSHHPEVPQRILRIMCRLEELGLAGRCLTLTPRPATEAELLTCHSA EYVGHLRATEKMKTRELHRESSNFDSIYICPSTFACAQLATGAACRLVEA VLSGEVLNGAAVVRPPGHAAEQDAACGFCFFNSVAVAARHAQTISGHALR ILIVDWDVHHGNGTQHMFEDDPSVLYVSLHRYDHGTFFPMGDEGASSQIG RAAGTGFTVNVAWNGPRMGDADYLAAWHRLVLPIAYEFNPELVLVSAGFD AARGDPLGGCQVSPEGYAHLTHLLMGLASGRIILILEGGYNLTSISESMA ACTRSLLGDPPPLLTLPRPPLSGALASITETIQVHRRYWRSLRVMKVEDR EGPSSSKLVTKKAPQPAKPRLAERMTTREKKVLEAGMGKVTSASFGEEST PGQTNSETAVVALTQDQPSEAATGGATLAQTISEAAIGGAMLGQTTSEEA VGGATPDQTTSEETVGGAILDQTTSEDAVGGATLGQTTSEEAVGGATLAQ TTSEAAMEGATLDQTTSEEAPGGTELIQTPLASSTDHQTPPTSPVQGTTP QISPSTLIGSLRTLELGSESQGASESQAPGEENLLGEAAGGQDMADSMLM QGSRGLTDQAIFYAVTPLPWCPHLVAVCPIPAAGLDVTQPCGDCGTIQEN WVCLSCYQVYCGRYINGHMLQHHGNSGHPLVLSYIDLSAWCYYCQAYVHH QALLDVKNIAHQNKFGEDMPHPH -
Predicted molecular weight
161 kDa including tags -
Amino acids
1 to 1215 -
Modifications
mutated H611A -
Tags
GST tag N-Terminus
-
Preparation and Storage
-
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.5
Constituents: 0.79% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.38% Potassium chloride, 0.79% Sodium chloride, 0.06% EDTA, 10% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
-
Functional Studies - Recombinant human HDAC6 (mutated H611A) protein (Active) (ab198058)
Activity assay using ab198058.
-
SDS-PAGE - Recombinant human HDAC6 (mutated H611A) protein (Active) (ab198058)4-20% SDS-PAGE analysis of 3 µg ab198058 with Coomassie staining.
