HDAC6 overexpression 293T lysate (whole cell) (ab94173)
Overview
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Product name
HDAC6 overexpression 293T lysate (whole cell)
See all HDAC6 lysates -
General notes
ab94173 is a 293T cell transfected lysate in which Human HDAC6 has been transiently over-expressed using a pCMV-HDAC6 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. Similarity: Belongs to the histone deacetylase family. HD type 2 subfamily. Contains 1 UBP-type zinc finger. PTM: Ubiquitinated. Its polyubiquitination however does not lead to its degradation. Sumoylated in vitro.
Images
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SDS-PAGE - HDAC6 overexpression 293T lysate (whole cell) (ab94173)ab94173 at 15µg/lane on an SDS-PAGE gel.
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Western blot - HDAC6 overexpression 293T lysate (whole cell) (ab94173)All lanes : Anti-HDAC6 antibody (ab56926) at 1/500 dilution
Lane 1 :HDAC6 overexpression 293T lysate (whole cell) (ab94173)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
