Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HDAC4 (phospho S246) + HDAC5 (phospho S259) + HDAC7 (phospho S155) antibody [EPR22997-46] (ab240643)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22997-46] to HDAC4 (phospho S246) + HDAC5 (phospho S259) + HDAC7 (phospho S155)
  • Suitable for: IP, WB, Dot blot
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-HDAC4 (phospho S246) + HDAC5 (phospho S259) + HDAC7 (phospho S155) antibody [EPR22997-46]

  • Description

    Rabbit monoclonal [EPR22997-46] to HDAC4 (phospho S246) + HDAC5 (phospho S259) + HDAC7 (phospho S155)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HeLa, whole, NIH/3T3, NIH/3T3, whole, C2C12, C2C12, whole, C6 and C6, whole lysates. IP: HeLa and C2C12 cells. Dot blot:

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-HDAC4 (phospho S246) + HDAC5 (phospho S259) + HDAC7 (phospho S155) antibody [EPR22997-46] (ab240643)
    Western blot - Anti-HDAC4 (phospho S246) + HDAC5 (phospho S259) + HDAC7 (phospho S155) antibody [EPR22997-46] (ab240643)
    All lanes : Anti-HDAC4 (phospho S246) + HDAC5 (phospho S259) + HDAC7 (phospho S155) antibody [EPR22997-46] (ab240643) at 1/1000 dilution

    Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate (untreated membrane)
    Lane 2 : C6, whole cell lysate (phosphatase treated membrane)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 121 kDa
    Observed band size: 124,140 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 15 seconds.

    The antibody detects endogenous level of HDAC4(140KDa), HDAC5(140KDa), HDAC7(124KDa) proteins only when phosphorylated on Ser246, Ser259 and Ser155, respectively.

  • Immunoprecipitation - Anti-HDAC4 (phospho S246) + HDAC5 (phospho S259) + HDAC7 (phospho S155) antibody [EPR22997-46] (ab240643)
    Immunoprecipitation - Anti-HDAC4 (phospho S246) + HDAC5 (phospho S259) + HDAC7 (phospho S155) antibody [EPR22997-46] (ab240643)

    HDAC5 (phospho S259) + HDAC4 (phospho S246) + HDAC7 (phospho S155) was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab240643 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab240643 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug

    Lane 2: ab240643 IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab240643 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 min.

     

  • Dot Blot - Anti-HDAC4 (phospho S246) + HDAC5 (phospho S259) + HDAC7 (phospho S155) antibody [EPR22997-46] (ab240643)
    Dot Blot - Anti-HDAC4 (phospho S246) + HDAC5 (phospho S259) + HDAC7 (phospho S155) antibody [EPR22997-46] (ab240643)

    Dot blot analysis of HDAC4 (phospho S246) + HDAC5 (phospho S259) + HDAC7 (phospho S155) labeled with ab240643 at 1/1000 dilution.

    Lane 1: HDAC5 (phospho S259) peptide (aa254-264).
    Lane 2: HDAC5 non-phospho peptide (aa251-264.
    Lane 3: HDAC7 (phospho S155) peptide (aa147-160).

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 15 seconds.

  • Western blot - Anti-HDAC4 (phospho S246) + HDAC5 (phospho S259) + HDAC7 (phospho S155) antibody [EPR22997-46] (ab240643)
    Western blot - Anti-HDAC4 (phospho S246) + HDAC5 (phospho S259) + HDAC7 (phospho S155) antibody [EPR22997-46] (ab240643)
    All lanes : Anti-HDAC4 (phospho S246) + HDAC5 (phospho S259) + HDAC7 (phospho S155) antibody [EPR22997-46] (ab240643) at 1/1000 dilution

    Lane 1 : C2C12 (mouse myoblasts myoblast), whole cell lysate (untreated membrane)
    Lane 2 : C2C12, whole cell lysate (phosphatase treated membrane)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 121 kDa
    Observed band size: 124,140 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 59 seconds.

    The antibody detects endogenous level of HDAC4(140KDa), HDAC5(140KDa), HDAC7(124KDa) proteins only when phosphorylated on Ser246, Ser259 and Ser155, respectively.

  • Western blot - Anti-HDAC4 (phospho S246) + HDAC5 (phospho S259) + HDAC7 (phospho S155) antibody [EPR22997-46] (ab240643)
    Western blot - Anti-HDAC4 (phospho S246) + HDAC5 (phospho S259) + HDAC7 (phospho S155) antibody [EPR22997-46] (ab240643)
    All lanes : Anti-HDAC4 (phospho S246) + HDAC5 (phospho S259) + HDAC7 (phospho S155) antibody [EPR22997-46] (ab240643) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate (untreated membrane)
    Lane 2 : NIH/3T3, whole cell lysate (phosphatase treated membrane)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 121 kDa
    Observed band size: 124,140 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 7 seconds.

    The antibody detects endogenous level of HDAC4(140KDa), HDAC5(140KDa), HDAC7(124KDa) proteins only when phosphorylated on Ser246, Ser259 and Ser155, respectively.

  • Western blot - Anti-HDAC4 (phospho S246) + HDAC5 (phospho S259) + HDAC7 (phospho S155) antibody [EPR22997-46] (ab240643)
    Western blot - Anti-HDAC4 (phospho S246) + HDAC5 (phospho S259) + HDAC7 (phospho S155) antibody [EPR22997-46] (ab240643)
    All lanes : Anti-HDAC4 (phospho S246) + HDAC5 (phospho S259) + HDAC7 (phospho S155) antibody [EPR22997-46] (ab240643) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate (untreated membrane)
    Lane 2 : HeLa whole cell lysate (phosphatase treated membrane)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 121 kDa
    Observed band size: 124,140 kDa why is the actual band size different from the predicted?



    Blocking and diution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    The antibody detects endogenous level of HDAC4(140KDa), HDAC5(140KDa), HDAC7(124KDa) proteins only when phosphorylated on Ser246, Ser259 and Ser155, respectively.

  • Anti-HDAC4 (phospho S246) + HDAC5 (phospho S259) + HDAC7 (phospho S155) antibody [EPR22997-46] (ab240643)
    Anti-HDAC4 (phospho S246) + HDAC5 (phospho S259) + HDAC7 (phospho S155) antibody [EPR22997-46] (ab240643)

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