Recombinant human PCSK9 (mutated D374T) protein (Active) (Biotin) (ab271652)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 85% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus, Avi tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
-
Product name
Recombinant human PCSK9 (mutated D374T) protein (Active) (Biotin)
See all PCSK9 proteins and peptides -
Biological activity
Reaction was performed using a PCSK9(D374T)-LDLR TR-FRET Assay Kit. Briefly, 5 ng of europium-labeled LDLR were mixed with varying amounts of PCSK9(D374T) and dye labeled acceptor in PCSK9 TR-FRET assay buffer and incubated at RT for 2 hr. Donor emission was read at 620 nm followed by acceptor emission at 665 nm.
-
Purity
>= 85 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
QEDEDGDYEELVLALRSEEDGLAEAPEHGTTATFHRCAKDPWRLPGTYVV VLKEETHLSQSERTARRLQAQAARRGYLTKILHVFHGLLPGFLVKMSGDL LELALKLPHVDYIEEDSSVFAQSIPWNLERITPPRYRADEYQPPDGGSLV EVYLLDTSIQSDHREIEGRVMVTDFENVPEEDGTRFHRQASKCDSHGTHL AGVVSGRDAGVAKGASMRSLRVLNCQGKGTVSGTLIGLEFIRKSQLVQPV GPLVVLLPLAGGYSRVLNAACQRLARAGVVLVTAAGNFRDDACLYSPASA PEVITVGATNAQDQPVTLGTLGTNFGRCVDLFAPGEDIIGASSDCSTCFV SQSGTSQAAAHVAGIAAMMLSAEPELTLAELRQRLIHFSAKDVINEAWFP EDQRVLTPNLVAALPPSTHGAGWQLFCRTVWSAHSGPTRMATAVARCAPD EELLSCSSFSRSGKRRGERMEAQGGKLVCRAHNAFGGEGVYAIARCCLLP QANCSVHTAPPAEASMGTRVHCHQQGHVLTGCSSHWEVEDLGTHKPPVLR PRGQPNQCVGHREASIHASCCHAPGLECKVKEHGIPAPQEQVTVACEEGW TLTGCSALPGTSHVLGAYAVDNTCVVRSRDVSTTGSTSEGAVTAVAICCR SRHLAQASQELQ -
Predicted molecular weight
74 kDa -
Amino acids
31 to 692 -
Modifications
mutated D374T -
Tags
His tag C-Terminus , Avi tag C-Terminus -
Additional sequence information
Signal peptide is removed.
-
-
Conjugation
Biotin
Preparation and Storage
-
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle. Store In the Dark.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
-
Functional Studies - Recombinant human PCSK9 (mutated D374T) protein (Active) (Biotin) (ab271652)Specific activity of ab271652.
-
SDS-PAGE - Recombinant human PCSK9 (mutated D374T) protein (Active) (Biotin) (ab271652)SDS-PAGE analysis of 2.2 µg ab271652.