Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant Human Carboxypeptidase A protein (His tag) (ab276282)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 97% SDS-PAGE
  • Endotoxin level:
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE

Description

  • Product name

    Recombinant Human Carboxypeptidase A protein (His tag)
    See all Carboxypeptidase A proteins and peptides

  • Purity

    > 97 % SDS-PAGE.

  • Endotoxin level

  • Expression system

    HEK 293 cells

  • Accession

    P15085

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MRGLLVLSVL LGAVFGKEDF VGHQVLRISV ADEAQVQKVK ELEDLEHLQL DFWRGPAHPG SPIDVRVPFP SIQAVKIFLE SHGISYETMI EDVQSLLDEE QEQMFAFRSR ARSTDTFNYA TYHTLEEIYD FLDLLVAENP HLVSKIQIGN TYEGRPIYVL KFSTGGSKRP AIWIDTGIHS REWVTQASGV WFAKKITQDY GQDAAFTAIL DTLDIFLEIV TNPDGFAFTH STNRMWRKTR SHTAGSLCIG VDPNRNWDAG FGLSGASSNP CSETYHGKFA NSEVEVKSIV DFVKDHGNIK AFISIHSYSQ LLMYPYGYKT EPVPDQDELD QLSKAAVTAL ASLYGTKFNY GSIIKAIYQA SGSTIDWTYS QGIKYSFTFE LRDTGRYGFL LPASQIIPTA KETWLALLTI MEHTLNHPY

    • Predicted molecular weight

      47 kDa including tags

    • Molecular weight information

      The secreted recombinant human CPA1 (pro form) consists of 414 amino acids and has a predicted molecular mass of 47 kDa. In SDS-PAGE under reducing conditions, it migrates with the apparent molecular mass of 43 kDa.

    • Amino acids

      1 to 419

    • Tags

      His tag C-Terminus

    • Additional sequence information

      Predicted N-terminus: Lys 17.

    • Specifications

    Our Abpromise guarantee covers the use of ab276282 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at Room Temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.40
      Constituent: 100% PBS

    • Reconstitution
      This information is lot specific. Please contact our technical Support team for details.

    General Info

    • Alternative names

      • Carboxypeptidase A1
      • carboxypeptidase A1 (pancreatic)
      • Carboxypeptidase A1 precursor
      • CBPA1_HUMAN
      • CPA
      • CPA1
      • Pancreatic carboxypeptidase A1
      • Procarboxypeptidase A1 pancreatic
      see all

    • Function

      Carboxypeptidase that catalyzes the release of a C-terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro.

    • Sequence similarities

      Belongs to the peptidase M14 family.

    • Cellular localization

      Secreted.
    • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human Carboxypeptidase A protein (ab276282)
    SDS-PAGE - Recombinant Human Carboxypeptidase A protein (ab276282)
    SDS-PAGE analysis of ab276282

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab276282? Please let us know so that we can cite the reference in this datasheet.

ab276282 has not yet been referenced specifically in any publications.

Preparation and Storage

  • Carboxypeptidase A1
  • carboxypeptidase A1 (pancreatic)
  • Carboxypeptidase A1 precursor

Images

  • SDS-PAGE - Recombinant Human Carboxypeptidase A protein (ab276282)
    SDS-PAGE - Recombinant Human Carboxypeptidase A protein (ab276282)
    SDS-PAGE analysis of ab276282

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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