Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)
![Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)](https://www.abcam.com/ps/products/181/ab181094/Images/ab181094-214730-anti-gcat-antibody-epr13450-n-terminal-western-blot.jpg "Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13450] to GCAT - N-terminal
- Suitable for: WB, ICC/IF, IP, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-GCAT antibody [EPR13450] - N-terminal
See all GCAT primary antibodies -
Description
Rabbit monoclonal [EPR13450] to GCAT - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanIP HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- BxPC-3, HepG2 and U87-MG cell lysates. Human fetal liver tissue lysate. Human heart and pancreas tissue. HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13450 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)All lanes : Anti-GCAT antibody [EPR13450] - N-terminal (ab181094) at 1/5000 dilution
Lane 1 : BxPC-3 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : U87-MG cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
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![Western blot - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)](https://www.abcam.com/ps/products/181/ab181094/Images/ab181094-214731-anti-gcat-antibody-epr13450-n-terminal-western-blot.jpg)
Western blot - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)Anti-GCAT antibody [EPR13450] - N-terminal (ab181094) at 1/5000 dilution + Human fetal liver lysate
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)](https://www.abcam.com/ps/products/181/ab181094/Images/ab181094-214726-anti-gcat-antibody-epr13450-n-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)Immunohistochemical analysis of Human heart tissue, staining GCAT with ab181094 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)](https://www.abcam.com/ps/products/181/ab181094/Images/ab181094-214727-anti-gcat-antibody-epr13450-n-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)Immunohistochemical analysis of Human pancreas tissue, staining GCAT with ab181094 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)](https://www.abcam.com/ps/products/181/ab181094/Images/ab181094-214725-anti-gcat-antibody-epr13450-n-terminal-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)Immunofluorescence analysis of HepG2 cells, staining GCAT (green) with ab181094 at 1/100 dilution. An Alexa Fluor®488-conjugated goat anti-rabbit IgG was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).
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![Immunoprecipitation - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)](https://www.abcam.com/ps/products/181/ab181094/Images/ab181094-214729-anti-gcat-antibody-epr13450-n-terminal-immunoprecipitation.jpg)
Immunoprecipitation - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate (lane 1) or negative control (lane 2), labeling GCAT immunoprecipitated using ab181094 at 1/40 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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![Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)](https://www.abcam.com/ps/products/181/ab181094/Images/ab181094-7-benefits-of-recombinant-antibodies.png)
Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)
