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Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13450] to GCAT - N-terminal
  • Suitable for: WB, ICC/IF, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-GCAT antibody [EPR13450] - N-terminal
    See all GCAT primary antibodies

  • Description

    Rabbit monoclonal [EPR13450] to GCAT - N-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • BxPC-3, HepG2 and U87-MG cell lysates. Human fetal liver tissue lysate. Human heart and pancreas tissue. HepG2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)
    Western blot - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)
    All lanes : Anti-GCAT antibody [EPR13450] - N-terminal (ab181094) at 1/5000 dilution

    Lane 1 : BxPC-3 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : U87-MG cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

  • Western blot - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)
    Western blot - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)
    Anti-GCAT antibody [EPR13450] - N-terminal (ab181094) at 1/5000 dilution + Human fetal liver lysate

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)

    Immunohistochemical analysis of Human heart tissue, staining GCAT with ab181094 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)

    Immunohistochemical analysis of Human pancreas tissue, staining GCAT with ab181094 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)
    Immunocytochemistry/ Immunofluorescence - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)

    Immunofluorescence analysis of HepG2 cells, staining GCAT (green) with ab181094 at 1/100 dilution. An Alexa Fluor®488-conjugated goat anti-rabbit IgG was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

  • Immunoprecipitation - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)
    Immunoprecipitation - Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)

    Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate (lane 1) or negative control (lane 2), labeling GCAT immunoprecipitated using ab181094 at 1/40 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)
    Anti-GCAT antibody [EPR13450] - N-terminal (ab181094)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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