Astana Biomed Group, an authorized Abcam distributor in Central Asia

Rat ALT ELISA Kit (ab234579)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 15.2 ng/ml
  • Range: 15.6 ng/ml - 1000 ng/ml
  • Sample type: EDTA Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Rat

Overview

  • Product name

    Rat ALT ELISA Kit
    See all Alanine Transaminase kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Rec protein 6 1.9%

  • Sample type

    Serum, EDTA Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    15.2 ng/ml

  • Range

    15.6 ng/ml - 1000 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 85 78% - 91%
    EDTA Plasma 101 90% - 143%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Rat

  • Product overview

    Rat ALT ELISA Kit (ab234579) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ALT protein in edta plasma and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat ALT with 15.2 ng/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Rat ALT Capture Antibody 1 x 600µl
    10X Rat ALT Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    Antibody Diluent 4BR 1 x 6ml
    Plate Seals 1 unit
    Rat ALT Lyophilized Recombinant Protein 2 vials
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.

  • Tissue specificity

    Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.

  • Pathway

    Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.

  • Sequence similarities

    Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Alternative names

    • AAT1
    • Alanine aminotransferase
    • Alanine aminotransferase 1
    • ALAT1_HUMAN
    • ALT1
    • Glutamate pyruvate transaminase 1
    • Glutamic alanine transaminase 1
    • Glutamic pyruvate transaminase (alanine aminotransferase)
    • Glutamic pyruvic transaminase 1
    • Glutamic--alanine transaminase 1
    • Glutamic--pyruvic transaminase 1
    • gpt
    • GPT 1
    • GPT1
    see all

  • Database links

    Images

    • Other - Rat ALT ELISA Kit (ab234579)
      Other - Rat ALT ELISA Kit (ab234579)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of rat ALT standard curve in Sample Diluent NS + Enhancer
      Example of rat ALT standard curve in Sample Diluent NS + Enhancer

      The ALT standard curve was prepared as described in Section 10.

    • Interpolated concentrations of recombinant ALT protein in rat serum and plasma-EDTA samples.
      Interpolated concentrations of recombinant ALT protein in rat serum and plasma-EDTA samples.

      The concentrations of ALT were measured in duplicates, interpolated from the ALT standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ALT concentration was determined to be 81.4 mg/mL in serum and 34.9 mg/mL in plasma-EDTA.

    • Sandwich ELISA - Rat ALT ELISA Kit (ab234579)
      Sandwich ELISA - Rat ALT ELISA Kit (ab234579)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Alternative products to Rat ALT ELISA Kit (ab234579)

    © 2021 Astana Biomed Group LLP. All rights reserved.