Recombinant Catalase protein (Active) (ab219092)
Key features and details
- Expression system: Escherichia coli
- Active: Yes
- Suitable for: Functional Studies
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Product name
Recombinant Catalase protein (Active)
See all Catalase proteins and peptides -
Biological activity
Specific activity: ≥ 8000 U/mg protein.
Unit definition: One unit is defined as the decomposition of 1μmol of H2O2 in 1 minute at 25°C at pH 7.0. The rate of disappearance of H2O2 is observed at 240 nm .
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Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Serratia -
Predicted molecular weight
220 kDa -
Additional sequence information
From Psychrotolerant Bacteria. Four subunits. Each subunit is ~ 55 kDa.
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Preparation and Storage
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Alternative names
- Cas1
- CAT
- CATA_HUMAN
see all -
Function
Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. -
Involvement in disease
Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions. -
Sequence similarities
Belongs to the catalase family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Peroxisome. - Information by UniProt
Images
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Functional Studies - Recombinant Catalase protein (Active) (ab219092)
pH dependence of ab219092. Activity was measured by monitoring pH from 3 to 11 at 25ºC.
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Functional Studies - Recombinant Catalase protein (Active) (ab219092)Temperature dependence of ab219092. Activity was measured by monitoring temperature from 20 to 80ºC using 50 mM potassium phosphate buffer (pH 7.0).
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Functional Studies - Recombinant Catalase protein (Active) (ab219092)Thermostability analysis of ab219092. Activity was monitored at 50°C using 50 mM potassium phosphate buffer (pH 7.0).
