Native human Catalase protein (ab91026)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
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Product name
Native human Catalase protein
See all Catalase proteins and peptides -
Biological activity
Activity: Greater than 50,000 units per mg protein. One unit is equal to the amount of enzyme that will decompose one micromole of hydrogen peroxide per minute at 25°C, pH 7.0 -
Purity
> 95 % SDS-PAGE. -
Expression system
Native -
Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Human -
Predicted molecular weight
230 kDa -
Additional sequence information
Purified from Human Erythrocytes. Four identical subunits each with a molecular weight of 58,000.
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Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituent: 0.79% Tris HClThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Native human Catalase protein (ab91026)SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
Lane 1: 5 μg Catalase (reduced/heated)
Lane 2: 10 μg Catalase (reduced/heated)
Lane 3: 20 μg Catalase (reduced/heated)
Lane 4: Molecular weight markers
Lane 5: 5 μg Catalase (non-reduced/no heat)
Lane 6: 10 μg Catalase (non-reduced/no heat)
Lane 7: 20 μg Catalase (non-reduced/no heat) -
Western blot - Native human Catalase protein (ab91026)All lanes : Anti-Catalase antibody - Peroxisome Marker (ab15834) at 5 µg/ml
Lane 1 :Native human Catalase protein (ab91026) at 0.1 µg
Lane 2 :Native human Catalase protein (ab91026) at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds
