HMG-CoA Reductase Activity Assay Kit (Colorimetric) (ab204701)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 10 min
- Sample type: Purified protein
Overview
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Product name
HMG-CoA Reductase Activity Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Purified protein -
Assay type
Enzyme activity -
Assay time
0h 10m -
Product overview
HMG-CoA Reductase Activity Assay Kit (Colorimetric) (ab204701) is suitable for measuring activity of purified HMG-CoA reductase or for screening inhibitors/activators of HMG-CoA reductase. It is based on the consumption of NADPH by the enzyme, which can be measured by the decrease of absorbance at OD=340 nm. The limit of detection is below 0.05 mU.
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Notes
HMG-CoA reductase (3-hydroxy-3-methyl-glutaryl-CoA reductase or HMGR) (EC 1.1.1.34) is the rate-controlling enzyme of the mevalonate pathway, the metabolic pathway that produces cholesterol from acetyl-CoA. In an NADPH-dependent reaction, HMG-CoA reductase reduces HMG-CoA to generate mevalonate and CoA. The enzyme is target of a group of cholesterol-lowering drugs known as statins. Inhibition of HMG-CoA reductase induces expression of LDL receptors in the liver, which lowers plasma concentration of cholesterol.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 100 tests HMG-CoA 1 vial HMG-CoA Reductase 1 vial HMG-CoA Reductase Assay Buffer 1 x 20ml Inhibitor (Atorvastatin, 10 mM) 1 x 10µl NADPH 1 vial -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
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Function
This transmembrane glycoprotein is involved in the control of cholesterol biosynthesis. It is the rate-limiting enzyme of sterol biosynthesis. -
Pathway
Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3. -
Sequence similarities
Belongs to the HMG-CoA reductase family. -
Cellular localization
Endoplasmic reticulum membrane. Peroxisome membrane. - Information by UniProt
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Alternative names
- 3 hydroxy 3 methylglutaryl CoA reductase
- 3 hydroxy 3 methylglutaryl Coenzyme A reductase
- 3 hydroxymethylglutaryl CoA reductase
see all
Images
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HMG-CoA Reductase Activity Assay Kit (Colorimetric)
Activity of HMG-CoA Reductase (positive control included in the kit) compared to the background control with no enzyme.
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HMG-CoA Reductase Activity Assay Kit (Colorimetric)Comparison of HMG-COA Reductase activity in absence or presence of specific inhibitors such as atorvastatin (100 µM) and pravastatin (10 ng/µl); ND: Not Detectable.