Rat TIMP1 ELISA Kit (ab100782)
Key features and details
- Sensitivity: 4 pg/ml
- Range: 4.1 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-rat TIMP1 2 vials Recombinant rat TIMP1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TIMP1 Microplate (12 x 8 wells) 1 unit TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. -
Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
Post-translational
modificationsThe activity of TIMP1 is dependent on the presence of disulfide bonds. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- Clgi
- Collagenase inhibitor
- Collagenase inhibitor, Human
see all -
Database links
- Entrez Gene: 116510 Rat
- SwissProt: P30120 Rat
- Unigene: 25754 Rat
Images
-
Typical data B
Representative standard curve using ab100782
-
Typical data ARepresentative standard curve using ab100782
