Rat Myoglobin ELISA Kit (ab260068)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.1 ng/ml
- Range: 0.47 ng/ml - 30 ng/ml
- Sample type: Cell culture media, Cell culture supernatant, EDTA Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat Myoglobin ELISA Kit
See all Myoglobin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 6.7% Inter-assay Sample n Mean SD CV% Serum 3 8.8% -
Sample type
Cell culture supernatant, Serum, Tissue Extracts, Cell culture media, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.1 ng/ml -
Range
0.47 ng/ml - 30 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 97 91% - 106% Serum 89 86% - 91% Tissue Extracts 109 103% - 117% Cell culture media 87 82% - 90% EDTA Plasma 106 95% - 119% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat -
Product overview
Rat Myoglobin ELISA Kit (ab260068) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Myoglobin protein in edta plasma, serum, cell culture media, tissue extracts, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Myoglobin with 0.1 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Myoglobin, is a 18.4 kDa heme protein, found in both cardiac and skeletal muscle that functions as a reserve supply of oxygen by facilitating the movement of oxygen within muscles. Damage to either type of muscle following conditions such as trauma, ischemia, and diseases that cause myopathy, is associated with the release of myoglobin into serum. Specifically, following cardiac necrosis associated with myocardial infarction (MI), myoglobin is one of the first markers to rise above normal levels. Myoglobin levels increase measurably above baseline within 2-4 hours post-infarct, peaking at 9-12 hours, and returning to baseline within 24-36 hours. Rat Myoglobin shares 83%, 93%, 77%, and 84% sequence homology with human, mouse, bovine, and monkey respectively.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat Myoglobin Capture Antibody 1 x 600µl 10X Rat Myoglobin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BR 1 x 6ml Plate Seals 1 unit Rat Myoglobin Lyophilized Recombinant Protein 2 vials Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. -
Sequence similarities
Belongs to the globin family. - Information by UniProt
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Alternative names
- MB
- MGC13548
- MYG_HUMAN
- Myoglobin
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Database links
- Entrez Gene: 59108 Rat
- SwissProt: Q9QZ76 Rat
- Unigene: 40511 Rat
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of rat Myoglobin standard curve in Sample Diluent NS.The Myoglobin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Myoglobin in rat serum and plasma (EDTA) samples.The concentrations of Myoglobin were measured in duplicates, interpolated from the Myoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100% and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Myoglobin concentration was determined to be 10.60 ng/mL in serum and 16.61 ng/mL in plasma (EDTA).
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Interpolated concentrations of native Myoglobin in rat heart tissue culture supernatant samples.The concentrations of Myoglobin were measured in duplicates, interpolated from the Myoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows: heart tissue culture supernatant 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Myoglobin concentration was determined to be 1927.15 ng/mL in heart tissue culture supernatant.
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Interpolated concentrations of native Myoglobin in rat heart extract and skeletal muscle extract based on a 50 ng/mL extract load.The concentrations of Myoglobin were measured in duplicate and interpolated from the Myoglobin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Myoglobin concentration was determined to be 5.28 ng/mL in heart extract and 5.87 ng/mL in skeletal muscle extract.
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Sandwich ELISA - Rat Myoglobin ELISA Kit (ab260068)To learn more about the advantages of recombinant antibodies see here.
