Rat Leptin ELISA Kit (ab229891)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 24 pg/ml
- Range: 47 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat Leptin ELISA Kit
See all Leptin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Rat serum 5 5.3% Inter-assay Sample n Mean SD CV% Rat serum 3 3.7% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
24 pg/ml -
Range
47 pg/ml - 3000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 106 100% - 106% Cell culture media 113 109% - 116% Hep Plasma 116 108% - 120% Cit plasma 111 109% - 113% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat
Does not react with: Cow -
Product overview
Rat Leptin ELISA Kit (ab229891) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Leptin protein in cell culture supernatant, cit plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Leptin with 24 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat Leptin Capture Antibody 1 x 600µl 10X Rat Leptin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Plate Seals 1 unit Rat Leptin Lyophilized Recombinant Protein 2 vials Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. -
Involvement in disease
Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. -
Sequence similarities
Belongs to the leptin family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- FLJ94114
- LEP
- LEP_HUMAN
see all -
Database links
- Entrez Gene: 25608 Rat
- SwissProt: P50596 Rat
- Unigene: 44444 Rat
Images
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Other - Rat Leptin ELISA Kit (ab229891)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Rat Leptin Competitor standard curve comparisonStandard curve comparison between Rat Leptin SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Interpolated concentrations of native Leptin in rat serum and plasma samples.The concentrations of Leptin were measured in duplicates, interpolated from the Leptin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Target concentration was determined to be 1889 pg/mL in serum, 1326 pg/mL in plasma (heparin) and 1026 pg/mL in plasma (citrate).
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Interpolated concentrations of spike Leptin in rat cell culture supernatant.The concentrations of Leptin were measured in duplicates, interpolated from the Leptin standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Example of rat Leptin standard curve.The Leptin standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.
