Rat Leptin ELISA Kit (ab100773)
Key features and details
- Sensitivity: 30 pg/ml
- Range: 10.97 pg/ml - 8000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Biotinylated anti-rat Leptin (lyophilized) 2 vials Leptin Microplate (12 x 8 wells) 1 unit Recombinant rat Leptin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. -
Involvement in disease
Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. -
Sequence similarities
Belongs to the leptin family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- FLJ94114
- LEP
- LEP_HUMAN
see all -
Database links
- Entrez Gene: 25608 Rat
- SwissProt: P50596 Rat
- Unigene: 44444 Rat
Images
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Sandwich ELISA - ab100773 Leptin Rat ELISA Kit
Rat Leptin measured in supernatanses showing quantity (ng) per mL of tested sample
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Sandwich ELISA - ab100773 Leptin Rat ELISA KitRat Leptin measured in biofluids showing quantity (ng) per mL of tested sample
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Sandwich ELISA - ab100773 Leptin Rat ELISA KitStandard curve: mean of duplicates (+/-SD) with background readings subtracted
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ELISA - Leptin Rat ELISA Kit (ab100773)
