Rat Fas Ligand ELISA Kit (CD95L) (ab100759)
Key features and details
- Sensitivity: 90 pg/ml
- Range: 82.3 pg/ml - 60000 pg/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 160X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-rat Fas Ligand 2 vials Fas Ligand Microplate (12 x 8 wells) 1 unit Recombinant Rat Fas Ligand Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
Fas / APO1 (CD95) is an important member of the tumour necrosis factor (TNF) superfamily involved in membrane-mediated apoptosis. Ligation of Fas by Fas Ligand or an anti Fas cross linking antibody, triggers activation of the caspase cascade. Functional impairment of the Fas / Fas Ligand system is associated with the development and progression of malignancies. Fas gene mutations have been suggested to have a role in testicular germ cell tumours. Tumour cells frequently exhibit de novo expression of Fas Ligand, which plays a significant role in local tissue destruction, metastatic spread, and immune escape of the tumor cells. The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/Fas Ligand system. -
Cellular localization
Cell Membrane (single-pass type II membrane protein) and Secreted -
Alternative names
- Apoptosis (APO 1) antigen ligand 1
- Apoptosis antigen ligand
- APT1LG1
see all -
Database links
- Entrez Gene: 25385 Rat
- SwissProt: P36940 Rat
Images
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Typical Standard Curve
Representative Standard Curve using ab100759.
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Typical Standard CurveRepresentative Standard Curve using ab100759.
