Rabbit monoclonal [RM117] Anti-Human IgG1 heavy chain (Biotin) (ab272183)
![Rabbit monoclonal [RM117] Anti-Human IgG1 heavy chain (Biotin) (ab272183)](https://www.abcam.com/ps/products/272/ab272183/Images/ab272183-385450-recombinant-Biotin-AntiHuman-IgG1-rabbit-monoclonal-antibody-RM117-titerelisa-human.png "Rabbit monoclonal [RM117] Anti-Human IgG1 heavy chain (Biotin) (ab272183)")
Key features and details
- Rabbit monoclonal [RM117] Anti-Human IgG1 heavy chain (Biotin)
- Conjugation: Biotin
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ELISA
Overview
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Product name
Rabbit monoclonal [RM117] Anti-Human IgG1 heavy chain (Biotin) -
Host species
Rabbit -
Target species
Human -
Tested applications
Suitable for: ELISAmore details -
Immunogen
Synthetic peptide corresponding to Human Human IgG (Hinge).
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
RM117 -
Isotype
IgG -
Research areas
Images
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ELISA - Rabbit monoclonal RM117 Anti-Human IgG1 heavy chain (Biotin) (ab272183)
A titer ELISA using ab272183. The plate was coated with different amounts of human IgG1. A serial dilution of ab272183 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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ELISA - Rabbit monoclonal RM117 Anti-Human IgG1 heavy chain (Biotin) (ab272183)ELISA of human immunoglobulins shows ab272183 only reacted to human IgG1. No cross reactivity with Human IgG2, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab272183 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Sandwich ELISA - Rabbit monoclonal RM117 Anti-Human IgG1 heavy chain (Biotin) (ab272183)Sandwich ELISA using ab272183 as the capture antibody, and a Biotinylated anti-human light chains (κ+ λ) antibody ab193185 as the detection antibody, followed by an AP conjugated streptavidin.
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Sandwich ELISA - Rabbit monoclonal RM117 Anti-Human IgG1 heavy chain (Biotin) (ab272183)Sandwich ELISA using ab272183 as the capture antibody, and a Biotinylated anti-human light chains (κ+ λ) antibody ab193185 as the detection antibody, followed by an AP conjugated streptavidin.