Protein Tyrosine Phosphatase Assay Kit (ab241032)
Key features and details
- Detection method: Fluorescent
Overview
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Product name
Protein Tyrosine Phosphatase Assay Kit -
Detection method
Fluorescent -
Product overview
Protein Tyrosine Phosphatase Assay Kit (ab241032) enables rapid measurement of protein tyrosine phosphatase (PTPase) activity, utilizing a fluorogenic protein phosphatase substrate that is converted into a highly fluorescent product (Ex/Em = 368/460 nm). A broad-spectrum PTPase inhibitor is provided for verification of specific activity in complex biological matrices, where serine/threonine phosphatases may contribute to substrate metabolism. Unlike ELISAs or malachite green-based approaches, the assay is homogeneous, continuous and does not require complicated sample processing or desalting to eliminate free phosphate. The assay is simple to perform, high-throughput adaptable and can detect a minimum of 0.1 mU PTPase activity.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Disulfide Reducing Agent (DTT) 1 x 300µl Fluorescence Standard 1 x 50µl PTPase Assay Buffer 1 x 25ml PTPase Inhibitor (Suramin) 1 vial PTPase Positive Control 1 vial PTPase Substrate 1 vial -
Research areas
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Function
Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Isoform 3 does not possess phosphatase activity. -
Tissue specificity
T-lymphocytes express only isoform 2. -
Sequence similarities
Belongs to the low molecular weight phosphotyrosine protein phosphatase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- Acid phosphatase 1 soluble
- Acid phosphatase of erythrocyte
- ACP1
see all
Images
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Fluorescence Standard curve.Fluorescence Standard curve. One mole of Fluorescence Standard corresponds to the metabolism of one mole of PTPase Substrate with release of one mole free inorganic phosphate.
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Reaction kinetics of PTPase Substrate metabolism.Reaction kinetics of PTPase Substrate metabolism in homogenates of human placenta (5 µg) and rat prefrontal cortex (10 µg) in the presence and absence of the PTPase inhibitor suramin.
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Quantification of PTPase activity in samples.Quantification of PTPase activity in samples (mean ± SEM of 4 or more independent replicates). Assays were performed according to the kit protocol.