Anti-SHP1 (phospho Y536) antibody (ab41437)
Key features and details
- Rabbit polyclonal to SHP1 (phospho Y536)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SHP1 (phospho Y536) antibody
See all SHP1 primary antibodies -
Description
Rabbit polyclonal to SHP1 (phospho Y536) -
Host species
Rabbit -
Specificity
Phospho-Tyr536-SHP1 specific. The antibody detects a 68 kDa band on SDS-PAGE immunoblots of human Jurkat cells treated with pervanadate (not observed in control cells). -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic phospho-Tyr536-SHP1 peptide (coupled to carrier protein) corresponding to amino acids around tyrosine 536 in human SHP1. The sequence is highly conserved in rat and mouse SHP1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was cross-adsorbed to a non specific phospho-tyrosine peptide then affinity purified using phospho-Tyr536-SHP1 peptide (immunogen). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SHP1 (phospho Y536) antibody (ab41437)Lanes 1-2 : anti-SHP1 (C-terminal)
Lanes 3-5 : Anti-SHP1 (phospho Y536) antibody (ab41437) at 1/1000 dilution
Lanes 1 & 3 : human Jurkat cells treated with 1 mM pervanadate for 30 min.
Lanes 2 & 4 : human Jurkat cells treated with 1 mM pervanadate for 30 min, blot exposed to alkaline phosphatase before probing
Lane 5 : human Jurkat cells treated with 1 mM pervanadate for 30 min.
with phospho-Tyr536-SHP1 peptide
Predicted band size: 68 kDa
Observed band size: 68 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
