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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-PRR11 antibody [OTI1A4] (ab131652)

Anti-PRR11 antibody [OTI1A4] (ab131652)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [OTI1A4] to PRR11
  • Suitable for: WB
  • Reacts with: Mouse, Dog, Human, African green monkey
  • Isotype: IgG1

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Overview

  • Product name

    Anti-PRR11 antibody [OTI1A4]
    See all PRR11 primary antibodies
  • Description

    Mouse monoclonal [OTI1A4] to PRR11
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Dog, Human, African green monkey
  • Immunogen

    Recombinant fragment corresponding to Human PRR11 aa 125-360. (NP_060774) produced in E.coli.
    Sequence:

    MPKFKQRRRKLKAKAERLFKKKEASHFQSKLITPPPPPPSPERVGISSID ISQSRSWLTSSWNFNFPNIRDAIKLWTNRVWSIYSWCQNCITQSLEVLKD TIFPSRICHRELYSVKQQFCILESKLCKLQEALKTISESSSCPSCGQTCH MSGKLTNVPACVLITPGDSKAVLPPTLPQPASHFPPPPPPPPLPPPPPPL APVLLRKPSLAKALQAGPLKKDGPMQITVKDLLTVKLKKTQSLDEKRKLI PSPKARNPLVTVSDLQHVTLKPNSKVLSTRVTNVLITPGKSQMDLRKLLR KVDVERSPGGTPLTNKENMETGTGLTPVMTQALRRKFQLAHPRSPTPTLP LSTSSFDEQN


    Database link: Q96HE9
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: HEK-293T cells transfected with pCMV6-ENTRY PRR11 cDNA; HepG2, HeLa, SVT2, A549, COS-7, jurkat, MDCK, PC-12 and MCF7 whole cell lysate.
  • General notes

    The clone number has been updated from 1A4 to OTI1A4, both clone numbers name the same clone.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Purified from cell culture supernatant by affinity chromatography.
  • Clonality

    Monoclonal
  • Clone number

    OTI1A4
  • Isotype

    IgG1
  • Research areas

    • Cancer
    • Signal transduction
    • Other
    • Cancer
    • Tumor biomarkers
    • Other

Images

  • Western blot - Anti-PRR11 antibody [OTI1A4] (ab131652)
    Western blot - Anti-PRR11 antibody [OTI1A4] (ab131652)
    All lanes : Anti-PRR11 antibody [OTI1A4] (ab131652) at 1/2000 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : SVT2 whole cell lysate
    Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
    Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 7 : MDCK whole cell lysate
    Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 9 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

    Predicted band size: 40 kDa

  • Western blot - Anti-PRR11 antibody [OTI1A4] (ab131652)
    Western blot - Anti-PRR11 antibody [OTI1A4] (ab131652)
    All lanes : Anti-PRR11 antibody [OTI1A4] (ab131652)

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY PRR11 cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 40 kDa



    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC201889)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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