Porcine IL-18 ELISA Kit (ab273203)
Key features and details
- Sensitivity: 80 pg/ml
- Range: 81.92 pg/ml - 20000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Pig
Overview
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Product name
Porcine IL-18 ELISA Kit
See all IL-18 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
80 pg/ml -
Range
81.92 pg/ml - 20000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 112.9 102% - 123% Plasma 119.1 114% - 125% Cell culture media 137.8 129% - 150% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Pig -
Product overview
Porcine IL-18 ELISA Kit (ab273203) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Porcine IL-18 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Porcine IL-18 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-18 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti- Porcine IL-18 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-18 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Porcine IL-18 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Porcine IL-18 Antibody 2 vials Porcine IL-18 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. -
Sequence similarities
Belongs to the IL-1 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Iboctadekin
- IFN gamma inducing factor
- IFN-gamma-inducing factor
see all -
Database links
Images
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Example data
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Porcine IL-18 ELISA kit (ab273203) Standard curve.
