Anti-CD40L antibody [AT161-10] (ab281905)
![Anti-CD40L antibody [AT161-10] (ab281905)](https://www.abcam.com/ps/products/281/ab281905/Images/ab281905-1-AntiCD40L-antibody-immunocytochemistry-peripheral-blood-monocytes-human.jpg "Anti-CD40L antibody [AT161-10] (ab281905)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [AT161-10] to CD40L
- Suitable for: Flow Cyt, ICC
- Reacts with: Human
Overview
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Product name
Anti-CD40L antibody [AT161-10] -
Description
Mouse monoclonal [AT161-10] to CD40L -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD40L. Raised by immunizing mice with Chinese hamster ovary (CHO) cells expressing human CD40L.
Database link: P29965 -
Positive control
- Flow cyt: Human peripheral blood leukocytes. ICC: Human peripheral blood monocyte cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
AT161-10 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry - Anti-CD40L antibody [AT161-10] (ab281905)
Immunofluorescence analysis of paraformaldehyde-fixed human peripheral blood monocytes labeling CD40L with the chimeric rabbit Iof this antibody at 10 µg/ml, followed by Alexa Fluor® 488 secondary antibody at 2 µg/ml. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom chimeric antibody, DAPI, merged channels and an isotype control. The isotype control was stained with Anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.
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![Flow Cytometry - Anti-CD40L antibody [AT161-10] (ab281905)](https://www.abcam.com/ps/products/281/ab281905/Images/ab281905-2-AntiCD40L-antibody-flowcytometry-peripheral-blood-leukocytes-human.jpg)
Flow Cytometry - Anti-CD40L antibody [AT161-10] (ab281905)Flow cytometric analysis of Human peripheral blood leukocytes labeling CD40L with an Anti-Fluorescein IgG antibody (isotype control, black line) or the rabbit chimera version of this antibody (blue line) at 1/100 dilution for 1 hour at room temperature. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at 1/1000 dilution and cells analysed using a flow-cytometer.
