Phosphoglucomutase Activity Assay Kit (Fluorometric) (ab211104)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell Lysate, Tissue Lysate
- Sensitivity: 20 µU
Overview
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Product name
Phosphoglucomutase Activity Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Cell Lysate, Tissue Lysate -
Assay type
Enzyme activity (quantitative) -
Sensitivity
20 µU -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Phosphoglucomutase Activity Assay Kit (Fluorometric) (ab211104) provides a sensitive, simple and convenient method for measuring Phosphoglucomutase (PGM) activity in plasma, cell and tissue lysates. The assay is based on the ability of Phosphoglucomutase to convert glucose-1-phosphate to glucose-6-phosphate. Glucose-6-phosphate is then oxidized by Glucose-6-phosphate dehydrogenase (G6PDH) to form NADPH, which in turn reduces the probe to generate an intense fluorescence product that can detected at Ex/Em = 535/587 nm.
This assay can detect as low as 20 µU of Phosphoglucomutase activity.
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Notes
Phosphoglucomutase (PGM, EC 5.4.2.2) facilitates the interconversion of glucose-1-phosphate (G1P) and glucose-6-phosphate (G6P). Glycogen breakdown generates G1P, which is converted to G6P by PGM. G6P can then go into the glycolytic pathway to generate ATP, or to the pentose phosphate pathway to generate ribose and NADPH. On the other hand, when cells have extra energy, G6P is converted by PGM into G1P to generate glycogen.
In humans, two isoforms have been described, PGM I and PGM II. PGM deficiency leads to glucose storage disease.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests NADPH Standard (0.2 µmol) 1 vial PGM Assay Buffer 1 x 25ml PGM Developer (30 U) 1 vial PGM Enzyme Mix (10 U) 1 vial PGM Positive Control (10 U) 1 vial PGM Substrate (10 µmol) 1 vial PicoProbe (in DMSO) 1 x 400µl -
Research areas
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Relevance
Phosphoglucomutase 5 is a phosphotransferase involved in the interconversion of glucose 1 phosphate and glucose 6 phosphate. This activity is essential in formation of carbohydrates from glucose 6 phosphate and in formation of glucose 6 phosphate from galactose and glycogen. -
Cellular localization
Cell junction, adherens junction. -
Alternative names
- Aciculin
- PGM RP
- PGMRP
see all
Images
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Typical NADPH Standard Calibration Curve.
PGM Activity Assay Kit (ab211104) Typical NADPH standard calibration curve.
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Phosphoglucomutase activityPGM Activity Assay Kit (ab211104) Kinetic curves showing PGM activity in positive control (PosCtrl, included in kit), Jurkat cell lysate (0.4 μg) and rat liver homogenate (0.2 μg). Curves of background control wells are also shown.
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Phosphoglucomutase specific activityPGM Activity Assay Kit (ab211104) Typical Phosphoglucomutase specific activity in Jurkat cell lysate (0.4 µg) and rat liver homogenate (0.2 µg).
