Phosphoglucomutase Activity Assay Kit (Colorimetric) (ab155896)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Plasma, Suspension cells, Tissue, Tissue Homogenate
- Sensitivity: 1 mU/well
Overview
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Product name
Phosphoglucomutase Activity Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Plasma, Tissue, Adherent cells, Suspension cells, Tissue Homogenate -
Assay type
Enzyme activity -
Sensitivity
Range
2.5 nmol/well - 12.5 nmol/wellSpecies reactivity
Reacts with: Mammals, Other speciesProduct overview
In Abcam’s Phosphoglucomutase Activity Assay Kit (Colorimetric) (ab155896), PGM (phosphoglucomuyase) converts glucose-1-phosphate to glucose-6-phosphate; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm. The Phosphoglucomutase Assay Kit is simple, sensitive and rapid and can detect PGM activity even less than 1 mU/reaction.
Notes
Phosphoglucomutase (PGM) plays a key role in carbohydrate metabolism and widely exists in all organisms. PGM interconverts Glucose- 1-Phosphate (G1P) and Glucose-6-Phosphate (G6P) depending on the body requirement. When glycogen breaks down, G1P is generated and phosphoglucomutase converts G1P to G6P, which can go either to glycolytic pathway to generate ATP, or to pentose phosphate pathway to generate ribose and NADPH. On the other hand, when cells have extra energy, PGM converts G6P to G1P, which generates glycogen. In humans, phosphoglucomutase have 2 isoforms (PGM I and PGM II). PGM deficiency leads to glucose storage disease. Detection of abnormal phosphoglucomutase activity is crucial for diagnosis, prediction and treatment of the disease.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.Platform
Microplate readerProperties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests NADH Standard Yellow 1 vial PGM Assay Buffer WM 1 x 27ml PGM Developer Red 1 vial PGM Enzyme Mix Green 1 vial PGM Positive Control Purple 1 vial PGM Substrate Blue 1 vial -
Research areas
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests NADH Standard Yellow 1 vial PGM Assay Buffer WM 1 x 27ml PGM Developer Red 1 vial PGM Enzyme Mix Green 1 vial PGM Positive Control Purple 1 vial PGM Substrate Blue 1 vial -
Research areas
Images
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ab155896 Phosphoglucomutase Activity Assay Kit (Colorimetric)
Quantity of phosphoglucomutase in mouse serum. To control for background signal in samples, substrate was excluded in teh control. Measurements from samples (diluted 2 times) were taken after 10 and 20 mintues, the increase in signal was used to calculate the activity (duplicates; +/- SD).
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ab155896 Phosphoglucomutase Activity Assay Kit (Colorimetric)Quantity of phosphoglucomutase in mouse kidney lysates, without prior ammonium sulfate precipitation. To control for background signal in samples, substrate was excluded in the control. The samples were tested in a range of different dilutions. Data shown is from 5 microgram kidney proteins. Measurements were taken after 10 and 20 mintues, the increase in signal was used to calculate the activity (duplicates; +/- SD).
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ab155896 Phosphoglucomutase Activity Assay Kit (Colorimetric)Quantity of phosphoglucomutase in positive control and mouse liver and kidney lysates (without prior ammonium sulfate precipitation). The samples were tested in a range of different dilutions. Data shown is from 1 microgram of liver proteins (in 50 uL) and 5 microgram of kidney proteins. Meauserments were taken after 10 and 20 mintues, the increase in signal was used to calculate the activity after correction for the sample input (duplicates; +/- SD).
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ab155896 Phosphoglucomutase Activity Assay Kit (Colorimetric)Standard curve with background signal subtracted (duplicates; +/- SD).
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NADH Standard CurveNADH standard curve produced using kit protocol.
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Phosphoglucomutase activity in positive controlsPhosphoglucomutase activity in pure PGM Positive Control, jurkat cell lysate (induced with 2 µM camptothecin) and bovine liver lysate respectively
