Cat IgG ELISA Kit (ab190523)
Key features and details
- Sensitivity: 4.1 ng/ml
- Range: 12.5 ng/ml - 800 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Cat
Overview
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Product name
Cat IgG ELISA Kit
See all IgG kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum Inter-assay Sample n Mean SD CV% Serum -
Sample type
Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
> 4.1 ng/ml -
Range
12.5 ng/ml - 800 ng/ml -
Recovery
> 85 %
Sample specific recovery Sample type Average % Range Serum > 85 12.5ng/ml - 800ng/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Cat -
Product overview
Abcam's IgG Cat ELISA Kit (ab190523) is an in vitro enzyme linked immunosorbent assay (ELISA) for the quantitative measurement of IgG in biological samples of cats.
In this assay the IgG present in samples reacts with the anti-IgG antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgG antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound IgG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo¬genic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml IgG Cat Antibody coated microwells 1 x 96 tests IgG Cat Calibrator (lyophilized) 1 vial IgG Cat HRP Conjugate 1 vial Stop Solution 1 x 12ml -
Research areas
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Cellular localization
Secreted -
Alternative names
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
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Images
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Standard Curve
Representative standard curve using ab190523 IgG Cat ELISA Kit