Ovine IL-12 p70 ELISA Kit (ab273255)
Key features and details
- Sensitivity: 2.5 pg/ml
- Range: 2.05 pg/ml - 200 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Sheep
Overview
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Product name
Ovine IL-12 p70 ELISA Kit
See all IL-12 p70 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.5 pg/ml -
Range
2.05 pg/ml - 200 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 72.35 68% - 79% Plasma 74.38 68% - 79% Cell culture media 94.78 80% - 114% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Sheep -
Product overview
Ovine IL-12 p70 ELISA Kit (ab273255) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Ovine IL-12 p70 in serum, plasma (Collect plasma using EDTA as an anticoagulant. Heparin and citrate are not recommended) and cell culture supernatants.
This assay employs an antibody specific for Ovine IL-12 p70 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-12 p70 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Ovine IL-12 p70 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-12 p70 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 150X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Anti-Ovine IL-12 p70 coated Microplate (12 x 8 wells) 1 unit Assay Diluent C 1 x 30ml Biotinylated Anti-Ovine IL-12 p70 Antibody 2 vials Ovine IL-12 p70 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Relevance
IL-12 p70 is a disulfide bonded heterodimer of the IL-12 40 kDa and 35 kDa subunits. -
Cellular localization
Secreted -
Alternative names
- IL12A
- IL12B
- CLMF p35
see all
Images
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Ovine IL-12 p70 ELISA Kit standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.