OliGlo™ TAMRA FISH Kit (ab253401)
Overview
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Product name
OliGlo™ TAMRA FISH Kit -
Product overview
Fluorescence in situ hybridization (FISH) kit which directly labels the phosphate groups (terminal and backbone) on DNA using a TAMRA labeling reagent prepared in situ, causing less disruption of hybridization and allowing labeling that is not sequence dependent.
This kit when used with the supplied standard labeling protocol will yield labeling efficiency of approximately 10 to 100 labels per kilobase of nucleic acid. The standard size kit is suitable for labeling up to 120 μg of nucleic acid. The trial size kit is suitable for labeling up to 35 μg of nucleic acid.
This kit is sold by Marker Gene under product code M1620/M1621.
Properties
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Storage instructions
Please refer to protocols. -
Components 120 µg 35 µg 3M Sodium Acetate 1 x 500µl 1 x 100µl Activating Resin 1 vial 1 vial Denaturation Buffer D 1 x 200µl 1 x 50µl DMF 1 x 200µl 1 x 50µl Hybridization Buffer H 1 x 750µl 1 x 150µl OliGlo™ TAMRA Labeling Dye Precursor 1 vial 1 vial Renaturation Buffer R 1 x 200µl 1 x 50µl TE Buffer 1 x 500µl 1 x 100µl -
Function
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. -
Tissue specificity
Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland. -
Sequence similarities
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
Contains 1 PDZ (DHR) domain. -
Domain
The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14. -
Post-translational
modificationsUbiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro). -
Cellular localization
Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines. - Information by UniProt
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Alternative names
- BNOS
- brain
- Constitutive NOS
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