N-Arachidonylglycine (NAGly), GPR18 agonist (ab120346)
Key features and details
- T-type Ca2+ channel blocker. Endogenous GLYT2 inhibitor.
- CAS Number: 179113-91-8
- Purity: > 98%
- Soluble in ethanol to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
N-Arachidonylglycine (NAGly), GPR18 agonist -
Description
T-type Ca2+ channel blocker. Endogenous GLYT2 inhibitor. -
Alternative names
- NAGly
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Biological description
Endogenous inhibitor of GLYT2 with little or no effect on GLYT1 and is analgesic in rat models of neuropathic and inflammatory pain. Anandamide-like compound, with little affinity at CB1 receptors, VR1 receptors and anandamide transporters. Potent inhibitor of T-type Ca2+ channels with little activity on anandamide-sensitive TRPV1 and TASK1 currents and high-voltage-activated (HVA) Cav1.2, Cav2.2, Nav1.7, and Nav1.8 currents.
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Purity
> 98% -
CAS Number
179113-91-8 -
Chemical structure
Images
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Functional Studies - N-Arachidonylglycine (NAGly), GPR18 agonist (ab120346)
ab32557 staining p38 (phospho T180 + Y182) in RAW 264.7 cells treated with N-Arachidonylglycine (ab120346), by ICC/IF. Increase in expression of p38 (phospho T180 + Y182) correlates with increased concentration of N-Arachidonylglycine, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120346 (N-Arachidonylglycine) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32557 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
