myo-Inositol Assay Kit (Fluorometric) (ab252896)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Cell Lysate, Other biological fluids, Tissue Lysate
Overview
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Product name
myo-Inositol Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Other biological fluids, Cell Lysate, Tissue Lysate -
Product overview
myo-Inositol Assay Kit (Fluorometric) (ab252896) gives the user a specific, rapid, high-throughput adaptable method for determination of this molecule in biological samples. A Sample Clean-Up Mix is provided for easy removal of glucose in the sample that may contribute to background. Our assay will quantify amounts of inositol as low as 20 pmoles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Inositol Assay Buffer 1 x 25ml Inositol Developer 1 vial Inositol Enzyme Mix 1 vial Inositol Standard 1 vial Probe (in DMSO) 1 x 400µl Sample Clean-Up Mix 1 vial
Images
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Myo-Inositol standard curve.
myo-Inositol standard curve.
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myo-Inositol content of pooled human serum.Serum was treated with Sample Clean-Up Mix and passed through a 10 kDa spin column and diluted 10-fold. 5-20 µL of the filtrate was run in the assay.
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myo-Inositol determination ion human breast milkHuman breast milk (20 µL) was diluted 5-fold with PBS and treated with Sample Clean-Up Mix, and after centrifugation, 2-8 µL of the filtrate was assayed.
