Mouse/Rat IgM ELISA Kit (ab215085)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 385 pg/ml
- Range: 1.56 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse, Rat
Overview
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Product name
Mouse/Rat IgM ELISA Kit
See all IgM kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.4% Inter-assay Sample n Mean SD CV% Serum 3 4.9% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
385 pg/ml -
Range
1.56 ng/ml - 100 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 105 91% - 118% Cell culture media 97 94% - 102% Hep Plasma 108 91% - 122% EDTA Plasma 100 89% - 107% Cit plasma 105 97% - 118% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse, Rat
Does not react with: Guinea pig, Cow -
Product overview
Mouse/Rat IgM ELISA Kit (ab215085) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IgM protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse/Rat IgM with 385 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Immunoglobulin M, or IgM, is an antibody produced by B cells and the largest of the immunoglobulins in the human circulatory system. IgM typically constitutes about 10% of serum immunoglobulins and is prominent in the early immune responses. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. IgM typically forms pentamers which includes the J chain, while in the less common hexameric form the J chain is absent. Although the common pentameric form of IgM contains 10 antigen binding sites, IgM cannot bind 10 antigens simultaneously due to size constraints. Class specific anti-immunoglobulin antibodies are useful for the characterization of malignant B cell proliferations. Most lymphocytic leukemias share either surface or intra cytoplasmic Ig with an isotypic restriction, which suggest the monoclonal nature of the cell population. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitt's lymphoma bear surface IgM, whereas plasmocytes from Waldenström's disease bear intracytoplasmic IgM.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BC 1 x 6ml 10X Mouse/Rat IgM Capture Antibody 1 x 600µl 10X Mouse/Rat IgM Detector Antibody 1 x 600µl Mouse/Rat IgM Lyophilized Purified Protein 2 vials Plate Seals 1 unit SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Sample Diluent NS (ab193972) 1 x 50ml Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as 'natural' blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences. Class specific anti immunoglobulin antibodies are useful for: The characterization of malignant B cell proliferations. All but acute lymphocytic leukemias share either surface or intra cytoplasmic Ig with an isotypic restriction, which suggest the monoclonal nature of the cell population. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitt's lymphoma bear surface IgM, whereas plasmocytes from Waldenström's disease bear intracytoplasmic IgM. The other isotypes are less frequently found. On the other hand multiple myelomas are usually of the IgG or IgA type. Characterization of plasma cells in inflammatory conditions: Plasma cell typing can be of use for the classification of intestinal inflammatory conditions such as inflammatory bowel disease and allergic conditions. In the latter a specific increase in the number of IgE plasma cells can be demonstrated. -
Cellular localization
Isoform 1: Secreted. During differentiation, B lymphocytes switch from expression of membrane bound IgM to secretion of IgM. Isoform 2: Cell membrane; Single pass type I membrane protein. -
Alternative names
- AGM1
- Constant region of heavy chain of IgM
- DKFZp686I15196
see all -
Database links
- Entrez Gene: 16019 Mouse
- Entrez Gene: 299357 Rat
- SwissProt: P01872 Mouse
- Unigene: 304472 Mouse
- Unigene: 342177 Mouse
- Unigene: 428442 Mouse
- Unigene: 435580 Mouse
- Unigene: 436922 Mouse
see all
Images
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Other - Mouse/Rat IgM ELISA Kit (ab215085)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse/rat IgM standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native IgM in mouse serum and plasma samplesThe concentrations of IgM were measured in duplicate, interpolated from the IgM standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:2,000, plasma (citrate) 1:1,000, plasma (EDTA) 1:2,000, and plasma (heparin) 1:2,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IgM concentration was determined to be 147.5 µg/mL in neat serum, 97.3 µg/mL in neat plasma (citrate), 136.5 µg/mL in neat plasma (EDTA) and 170.6 µg/mL in neat plasma (heparin).
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Interpolated concentrations of native IgM in rat serum and plasma samples.The concentrations of IgM were measured in duplicates, interpolated from the IgM standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:8,000, and plasma (citrate) 1:8,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IgM concentration was determined to be 614.5 µg/mL in neat serum and 455.0 µg/mL in neat plasma (citrate).
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A comparison of mouse, rat, and human IgM proteins is shown.The human and mouse/rat IgM curves are parallel, but the human IgM curve is shifted to the left by a factor of 2.3 compared to the mouse/rat IgM curve.
