Mouse XCL1 Antibody Pair - BSA and Azide free (ab270377)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Mouse
Overview
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Product name
Mouse XCL1 Antibody Pair - BSA and Azide free
See all XCL1 kits -
Assay type
ELISA set -
Range
18.75 pg/ml - 1200 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
The Antibody Pair can be used to quantify Mouse XCL1. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab269540), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Mouse XCL1 Capture Antibody (unconjugated) 1 x 100µg Mouse XCL1 Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Relevance
Human lymphotactin (Lptn)/XCL1, also named human SCM1 and ATAC, is a member of gamma or C subfamily of chemokines that lack two of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended C-terminus. Human lymphotactin encodes a 114 amino acid precursor protein with a 21 amino acid predicted signal peptide. The gene for human and mouse lymphotactin has been mapped to chromosome 1. Human and mouse lymphotactin share approximately 60% amino acid sequence identity. The expression of lymphotactin is restricted to activated T cells such as activated CD8+ T cells and other class I MHC restricted T cells. Since lymphotactin is produced by lymphocytes and acts on lymphocytes, it is speculated that it is a messenger in T cell chemoattraction. Human lymphotactin is chemotactic for lymphocytes and NK cells. Its expression is abundant in some activated T cells such as activated CD8+ T cells and other class I MCH restricted T cells and is absent in CD4+ T cells. It has been shown that lymphotactin is a key regulator of lymphocyte motility and adhesion during acute allograft rejection. The specific receptor for lymphotactin is the orphan receptor XCR1 (GPR5). It has chemotactic activity for lymphocytes and natural killer cells but not for monocytes or neutrophils. -
Cellular localization
Secreted -
Alternative names
- ATAC
- Chemokine (C motif) ligand 1
- Cytokine SCM1
see all -
Database links
- Entrez Gene: 16963 Mouse
- SwissProt: P47993 Mouse
Images
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Sandwich ELISA - Mouse XCL1 Antibody Pair - BSA and Azide free (ab270377)To learn more about the advantages of recombinant antibodies see here.
