Mouse TrkB Matched Antibody Pair Kit (ab218169)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 250 pg/ml - 16000 pg/ml
Overview
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Product name
Mouse TrkB Matched Antibody Pair Kit
See all TrkB kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
24.18 pg/ml -
Range
250 pg/ml - 16000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse TrkB Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse TrkB.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab203368. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse TrkB Capture Antibody 2 x 50µg 1 x 50µg Mouse TrkB Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse TrkB Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1. -
Tissue specificity
Isoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsLigand-mediated auto-phosphorylation. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- AI848316
- BDNF tropomyosine receptor kinase B
- BDNF/NT 3 growth factors receptor
see all -
Database links
- Entrez Gene: 18212 Mouse
- SwissProt: P15209 Mouse
- Unigene: 130054 Mouse
Images
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Mouse TrkB standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse TrkB Matched Antibody Pair Kit (ab218169)To learn more about the advantages of recombinant antibodies see here.
