Anti-SF3B4 antibody (ab157117)
Key features and details
- Rabbit polyclonal to SF3B4
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SF3B4 antibody
See all SF3B4 primary antibodies -
Description
Rabbit polyclonal to SF3B4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
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Immunogen
Synthetic peptide corresponding to a region within amino acids 374-424 of Human SF3B4 (NP_005841.1).
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Positive control
- WB: HeLa, 293T, NIH/3T3 and Jurkat whole cell lysate. IP: 293T cell.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SF3B4 antibody (ab157117)All lanes : Anti-SF3B4 antibody (ab157117) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 44 kDa
Exposure time: 10 seconds
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Immunoprecipitation - Anti-SF3B4 antibody (ab157117)Immunoprecipitation analysis of whole cell lysate from 293T cells (1 mg for IP; 20% of IP loaded), labeling SF3B4 with ab157117 at 6 µg/mg lysate (lane 2). Immunoprecipitation of SF3B4 was also tested using an alternative Rabbit anti-SF3B4 (lane 1), or control IgG (lane 3). For blotting immunoprecipitated SF3B4, ab157117 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
