Recombinant human NGF protein (Animal Free) (ab217472)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, HPLC
-
Product name
Recombinant human NGF protein (Animal Free)
See all NGF proteins and peptides -
Biological activity
Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.
-
Purity
> 98 % SDS-PAGE.
> 98 % by HPLC. -
Expression system
Escherichia coli -
Accession
-
Protein length
Protein fragment -
Animal free
Yes -
Nature
Recombinant -
-
Species
Human -
Sequence
SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVR -
Predicted molecular weight
13 kDa -
Amino acids
122 to 239 -
Additional sequence information
A non-covalently-linked homodimer of two 13.5 kDa, polypeptide monomers that each contain 120 amino acids and three disulfide bonds.
-
Preparation and Storage
-
Alternative names
- Beta nerve growth factor
- Beta NGF
- Beta-nerve growth factor
see all -
Function
Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177). -
Involvement in disease
Neuropathy, hereditary sensory and autonomic, 5 -
Sequence similarities
Belongs to the NGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
