Anti-GDNF Receptor alpha 2/GFRA2 antibody (ab205351)
Key features and details
- Chicken polyclonal to GDNF Receptor alpha 2/GFRA2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgY
Overview
-
Product name
Anti-GDNF Receptor alpha 2/GFRA2 antibody
See all GDNF Receptor alpha 2/GFRA2 primary antibodies -
Description
Chicken polyclonal to GDNF Receptor alpha 2/GFRA2 -
Host species
Chicken -
Specificity
ab205351 does not recognizes hGFRa-1, hGFRa-3, hGFRa-4. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
-
Immunogen
Recombinant full length protein (His-tag) corresponding to Human GDNF Receptor alpha 2/GFRA2. Produced using CHO suspension cell line, and then purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
Database link: O00451 -
Positive control
- U2OS cells; His tagged GDNF Receptor alpha 2/GFRA2 recombinant protein.
-
General notes
This product was previously labelled as GDNF Receptor alpha 2
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: 100% PBS
As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-GDNF Receptor alpha 2/GFRA2 antibody (ab205351)
Immunofluorescent analysis of U2OS cells labeling GDNF Receptor alpha 2/GFRA2 using ab205351 at 1/2000. Goat anti-chicken IgY - H&L DyLight 550 (ab96952) at 1/200 dilution was used as secondary antibody. For nuclear staining DAPI was used.
-
Western blot - Anti-GDNF Receptor alpha 2/GFRA2 antibody (ab205351)Anti-GDNF Receptor alpha 2/GFRA2 antibody (ab205351) at 5000 µg/ml + His tagged GDNF Receptor alpha 2/GFRA2 at 0.1 µg
Secondary
Rabbit anti-chicken
Predicted band size: 51 kDa
